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Multimodal imaging made easy with LEGO
The Henriques Lab has听developed a new bit of hardware called NanoJ-Fluidics (also known as Pumpy McPumpface in less formal occasions). NanoJ-Fluidics is a fluidic exchange system that contains a syringe pump array made of LEGO and is controlled via the popular software packages ImageJ, FIJI or MicroManager.
Pumpy enables automated sample fluid exchange on the microscope, allowing complex workflows to be streamlined without the need for any specialised equipment, consumables or user hands-on engagement. A preprint in BioRxiv听showcases what Pumpy can do by performing live-to-fixed correlative imaging, multi-step staining protocols and multi-modal super-resolution microscopy - all听without ever removing the sample from the microscope and with virtually no user involvement.听
Pumpy is fun and inexpensive to build, as well as easy-to-use. It is fully open-source;听instructions听on how to replicate Pumpy听and听get your own Pumpy pumping听can be found听in the Henriques Lab听.
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