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Cellular decision-making
The primary interest of our laboratory is to understand how cells make decisions about their identity during development. Despite the intense activity in the field of developmental biology for many decades, our understanding of how cells make decisions is decidedly limited. Models tend to fall into two categories鈥� instructive or permissive. Instructive models emphasise the external or inherited trigger, giving the cell no choice. Permissive models view the cell as waiting for some event that triggers a decision already made, often incorporating a central 鈥渘oise plus feedback鈥� element. These models can be useful in the limited number of apparently simple contexts in which cell fates appear to be determined by a limited number of components, yet in most systems, which are neither wholly instructive nor permissive, and often depend on a highly complex set of interactions between the cell and its environment, these opposing models lack utility.
To allow a deeper understanding of cell decision making, and the generation of more useful conceptual frameworks, we have developed and implemented a range of technologies- with the view that to understand the decision of cell requires monitoring a broad range of regulatory features of single cells, as they differentiate. These approaches encompass both imaging and transcriptomic methods for monitoring the signalling 听and gene expression dynamics of single cells. In particular, we use imaging to directly observe the bursts of activity of individual genes in living cells . This approach provides a real-time readout of the gene expression decisions of the cell, in parallel with other features of the cells and their environment. We combine these techniques with detailed quantitative analysis of the data, and use modelling and molecular genetics to refine our hypotheses.
