Electron Microscopy

The LMCB has been specialising in cell biological EM for over 20 years. The facility comprises state-of-the-art specimen preparation and imaging technologies which, together with two experienced researchers, support the needs of the LMCB groups in all their EM research. 听

With experience in a wide range of electron microscopy techniques as well as a broad range of model organisms, we can provide advice, training and practical support for researchers throughout the entire timeline of their projects. For example: planning experiments, sample preparation, imaging and data analysis. The support given is tailored to each project, and can vary from advice and technical support for the more experienced, full training in required techniques and the equipment use for the less experienced researchers or the undertaking of the EM projects ourselves, a collaborative endeavour as part of the ongoing research.

Selected publications

White IJ and Burden JJ (2023).听. Methods Cell Biol. 2023;177:171-196.听Link to PDF.

White IJ, et al (2023),听听(Science/AAAS, Washington, DC, 2022), p. 6-8

Roubinet C, et al (2021).听. Curr Biol. Sep 27;31(18):3973-3983

Lopes da Silva M, et al (2019).听. Dev Cell. 2019 Jun 3;49(5):786-801.e6. doi: 10.1016/j.devcel.2019.04.006.

Russell MR, et al (2017).听. J Cell Sci. Jul 21. pii: jcs.188433

Equipment

Electron Microscopes

ZEISS FEG Scanning Electron Microscope with Array Tomography: Zeiss Gemini 300 SEM
Secondary electron detectors
Retractable Backscatter electron detector
STEM detector
Atlas 5 with Scan generator
Tandem decel sample bias
Variable pressure (and nanoVP)

FEI 120kV Transmission Electron Microscope: FEI 120kV Tecnai
Compustage
Tilt tomography
Fischione holders
OSIS digital camera
Gatan digital camera