Training:
Training is required before using facility equipment, please register and request training on .
Training is free for ICH / GOSH users. £50 per hour for other UCL users.
Fluorescence imagingÌý-ÌýBook onÌý.
- #1 Leica upright manualÌý3 colour fluorescence scope with IDS 3260CP monochrome camera. High NA objectives (10x/0.4, 20x/0.7, 63x/1.2Water, 63x/1.4Oil, 100x/1.4).ÌýGood for slides.ÌýLink toÌýuser guide.
- #3 Zeiss Observer inverted fully automated 7Ìýcolour fluorescence ().ÌýHamamatsu Flash 4v3 camera, HighÌýNA objectives (5x/0.16 10x/0.45 20x/0.8 40x/1.3 63x/1.4).ÌýGreat for slides, tilescans, z-stacks, deconvolution.ÌýLink to user guide.Ìý
- Olympus IX71 inverted TC scopeÌýwith Hamamatsu Orca R2 monochrome camera. For cells in TC plates flasks and dishes.ÌýLink to user guide.
Brightfield imaging (i.e H&E, DAB etc) -ÌýBook onÌý.
Scope 2 can also do fluorescecne, but the scopes above are far better for fluorescence. These scopes are best for colour imaging of histology slides.
- #2 Zeiss Axioplan2 - Zeiss Axiocam HRc colour camera (donated by NDU). Polarization contrast scope, see image below.
- #5 Zeiss Axioplan2 - Zeiss Axiocam HRc colour camera (donated by MMU/NU).
- Link to Kohler set up scope #2Ìý(microscope set up) & Link to software guide.
Location:Ìý
Widefield imaging facilities are on the 3rd floor Main Building, rooms 310, 301 and 304.Ìý
Operating instructions:
Follow links above. Also available:Ìýpixel sizes for scale bars and details of filter sets areÌýin theÌýAdvice and ProtocolsÌýpages.
Charges:Ìý
Picrosirius Red staining of collagen fibres under normal and polarized light.